Q141K polymorphism of ABCG2 protein is associated with poor prognosis in adult acute myeloid leukemia treated with idarubicin-based chemotherapy.

Overexpression of multidrug resistance protein ABCG2 has been associated to chemotherapy failure in solid and hematologic tumors. More than 40 ‘synonymous’ and ‘non-synonymous’ single nucleotide polymorphisms (SNPs) of ABCG2 have been identified. Among them, the 421C>A (Q141K), that is also the most common in Caucasian ethnicity (around 10%) has been shown to alter protein function and to modify in vitro sensitivity to many anticancer drugs, including classical anthracyclines and mitoxantrone. A recent report on the prognostic value of six ABCG2 gene polymorphisms in 184 Chinese acute leukemia patients seems to suggest that the presence of Q141K is associated with worse outcome. The aim of this study was to evaluate the frequency of the Q141K variant in a cohort of Caucasian patients with acute myeloid leukemia (AML) and to assess the impact of this polymorphism on the outcome of a treatment strategy that included idarubicin as the only anthracycline. One hundred and sixty-three consecutive patients with non-promyelocytic AML were included in the study. Leukemic cells were collected during diagnostic procedure after obtaining informed consent. One hundred and twenty-five patients received chemotherapy according to the institutional protocols active at diagnosis, including an induction course with FLAI/FLAIE regimen and at least one consolidation course with high-dose cytarabine, as previously described. Idarubicin was the only anthracycline used through the entire chemotherapy program. ABCG2 expression was studied on blast cells by flow cytometry, as previously described. ABCG2 genotype was analyzed with the TaqMan SNP Q141K assay (Applied Biosystems, C_15854163_70) and the test was performed in duplicate. Q141K polymorphism was detected in 29 of 163 patients (18%), and 2 patients were homozygous for the mutation. Of the 125 patients receiving chemotherapy, 26 displayed Q141K variant while 99 were ABCG2 wt. Table 1 summarizes the characteristics of patients with or without ABCG2 polymorphism; no significant differences were observed in the clinical or biological features of the two cohorts, also when taking ABCG2 expression into consideration. Seventy-three out of 125 patients (58%) attained complete remission (CR) after induction therapy with FLAI/FLAIE. No impact on CR was demonstrated for ABCG2 overexpression and Q141K polymorphism. Twenty-seven of 73 (37%) patients relapsed after a median time of 26 months. A shorter disease-free survival (DFS) was associated with CD56 aberrant expression, CD34 positivity and ABCG2 overexpression. Again, ABCG2 Q141K polymorphism had no impact on relapse; among Q141K patients, 7 of 16 (44%) relapsed while 20 of the 57 patients (35%) with wt ABCG2 who attained CR relapsed; 3-year DFS was 47% [CI: 30-64] in Q141 cases, and 61% [CI: 47-75] in wt patients (P=0.36). However, when patients were stratified in three groups according to ABCG2 expression (low or high) and presence of Q141K polymorphism, a negative impact of Q141K emerged. As shown in Figure 1A, DFS in Q141K patients was similar to that of cases with wt ABCG2 but protein overexpression (14 of 27 patients relapsed, 52%; 3-year DFS 44%, CI: 23-65). This was significantly shorter than in patients with